Indole production by organisms is observed by inserting the Kovac's reagent strip between the plug and inner wall of the tube, above the inoculated Peptone Water and incubating at 35-37°C for 18-24 hours. Preparation of Kovac's reagent. Kovac's reagent is prepared by dissolving 10 gm of p-dimethyl aminobenzaldehyde in 150 ml of isoamyl alcohol and then slowly adding 50 ml of concentrated hydrochloric acid.
Indole production by organisms is observed by inserting the Kovac's reagent strip between the plug and inner wall of the tube, above the inoculated Peptone Water and incubating at 35-37°C for 18-24 hours. Preparation of Kovac's reagent. Kovac's reagent is prepared by dissolving 10 gm of p-dimethyl aminobenzaldehyde in 150 ml of isoamyl alcohol and then slowly adding 50 ml of concentrated hydrochloric acid.
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