Prepare Blood Agar (TM 473) plates and inoculate the plates with suspect organism by surface spreading technique to obtain confluent growth. Place the Bacitracin Disks aseptically onto the inoculated surface and press gently. Invert the plates and incubate at 35-37°C in 5-10% CO2 for 18-24 hours till growth appears. Observe for the zone of inhibition around the disc. A zone of inhibition greater than or equal to 14 mm indicates susceptibility to bacitracin and is presumptive of group A streptocci. For further confirmation serological grouping should be performed
Prepare Blood Agar (TM 473) plates and inoculate the plates with suspect organism by surface spreading technique to obtain confluent growth. Place the Bacitracin Disks aseptically onto the inoculated surface and press gently. Invert the plates and incubate at 35-37°C in 5-10% CO2 for 18-24 hours till growth appears. Observe for the zone of inhibition around the disc. A zone of inhibition greater than or equal to 14 mm indicates susceptibility to bacitracin and is presumptive of group A streptocci. For further confirmation serological grouping should be performed
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