MacCONKEY AGAR (W/O CV, W/ 0.15% BILE SALTS & NaCI)
-Dissolve 51.53 gm of medium in 1000 ml purified/distilled water.
-Heat to boiling with gentle swirling to dissolve the agar completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 45 - 50°C. Mix well and pour into sterile Petri plates. (The surface of the medium should be dry when
inoculated).
-Dissolve 51.53 gm of medium in 1000 ml purified/distilled water.
-Heat to boiling with gentle swirling to dissolve the agar completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 45 - 50°C. Mix well and pour into sterile Petri plates. (The surface of the medium should be dry when
inoculated).
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