MacCONKEY AGAR (W/ SODIUM TAUROCHOLATE W/O CV & NaCl.)
-Dissolve 55.0 grams of medium in 1000 ml purified/distilled water.
-Heat to boiling with gentle swirling to dissolve the agar completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 45-50°C.Mix well and pour into sterile Petri plates.
-The surface of the medium should be dry when inoculated.
-Dissolve 55.0 grams of medium in 1000 ml purified/distilled water.
-Heat to boiling with gentle swirling to dissolve the agar completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes. Avoid overheating.
-Cool to 45-50°C.Mix well and pour into sterile Petri plates.
-The surface of the medium should be dry when inoculated.
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