MacCONKEY AGAR (W/ 0.15% BILE SALTS, CV & NaCl)
-Dissolve 51.53 grams in 1000 ml purified/ distilled water.
-Heat to boiling with gentle swirling to dissolve the agar completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Avoid overheating. Cool to 45 - 50°C and pour into sterile Petri plates.
-The surface of the medium should be dry when inoculated.
-Dissolve 51.53 grams in 1000 ml purified/ distilled water.
-Heat to boiling with gentle swirling to dissolve the agar completely.
-Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
-Avoid overheating. Cool to 45 - 50°C and pour into sterile Petri plates.
-The surface of the medium should be dry when inoculated.
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