FLUID TETRATHIONATE MEDIUM W/O IODINE AND BG (TETRATHIONATE BROTH BASE W/O IODINE & BG)
-Suspend 46.0 grams in 1000 ml purified/distilled water and heat just to boiling.
-DO NOT AUTOCLAVE. Cool below 45°C and add 20 ml iodine solution (iodine - 6 grams and potassium iodide - 5 grams in 20 ml distilled water) and 10 ml of 0.1% brilliant green solution.
-Mix well and dispense in 10 ml quantities. This complete medium should be used on the day of preparation otherwise sterilized broth base may be stored for some time.
-Do not heat after the addition of iodine solution. Use the medium immediately after addition of iodine.
-Suspend 46.0 grams in 1000 ml purified/distilled water and heat just to boiling.
-DO NOT AUTOCLAVE. Cool below 45°C and add 20 ml iodine solution (iodine - 6 grams and potassium iodide - 5 grams in 20 ml distilled water) and 10 ml of 0.1% brilliant green solution.
-Mix well and dispense in 10 ml quantities. This complete medium should be used on the day of preparation otherwise sterilized broth base may be stored for some time.
-Do not heat after the addition of iodine solution. Use the medium immediately after addition of iodine.
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