-Dissolve 40.01 grams in 1000 ml purified/distilled water. -Heat if necessary to dissolve the medium and distribute into test tubes with inverted Durham tubes. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.=Yes
-Dissolve 40.01 grams in 1000 ml purified/distilled water. -Heat if necessary to dissolve the medium and distribute into test tubes with inverted Durham tubes. -Sterilize by autoclaving at 15 psi pressure (121°C) for 15 minutes.
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