DECARBOXYLASE TEST MEDIUM BASE (FALKOW) (as per BIS)
-Dissolve 9.00 grams in 1000ml distilled water. Gently heat, if necessary to dissolve the medium completely. -Divide into four equal parts. One part is tubed without addition of any amino acid. To the remaining three parts, add separately L-lysine hydrochloride
-Dissolve 9.00 grams in 1000ml distilled water. Gently heat, if necessary to dissolve the medium completely. -Divide into four equal parts. One part is tubed without addition of any amino acid. To the remaining three parts, add separately L-lysine hydrochloride, L-arginine hydrochloride and L-ornithine hydrochloride to a fil concentration of 0.5%. -Add 0.5% amino acid (L – Lysine or L – Arginine or L - Ornithine) and agitate to dissolve completely. -Dispense 5 ml amounts into screw capped test tubes. -Sterilize by autoclaving at 10 psi (115°C) for 20 minutes. -Cool at room temperature prior to use.
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