CHROMOGENIC LISTERIA AGAR BASE (Modified) (as per ISO 11290:2004) (Store below 8° C)
-Dissolve 70.05 gm in 970 ml of distilled water. -Gently heat to boiling with gentle swirling to dissolve the agar completely. -Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. -Cool to 45 – 50°C. -Rehydrate contents of 2 vials of Chromogenic Listeria Selective Supplement (TS 205) in 5 ml distilled water and add aseptically to the molten medium
-Dissolve 70.05 gm in 970 ml of distilled water. -Gently heat to boiling with gentle swirling to dissolve the agar completely. -Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. -Cool to 45 – 50°C. -Rehydrate contents of 2 vials of Chromogenic Listeria Selective Supplement (TS 205) in 5 ml distilled water and add aseptically to the molten medium, along with contents of 2 vials of Chromogenic Enrichment Supplement (TS 031). -Mix well and pour into sterile Petri plates.
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